Analysis of Aluminum Stress-induced Differentially Expressed Proteins in Alfalfa Roots Using Proteomic Approach

서지정보

ㆍ발행기관 : 한국초지조사료학회 ㆍ수록지정보 : 한국초지조사료학회지 / 42권 / 3호
ㆍ저자명 : Dong-Hyun Kim, Joon-Woo Lee, Chang-Woo Min, Md. Atikur Rahman, Yong-Goo Kim, Byung-Hyun Lee

목차

ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
1. Plant growth and aluminum treatments
2. Protein extraction, 2DE and gel image analysis
3. In-gel digestion and MALDI-TOF/TOF MS
4. RNA extraction and RT-PCR
5. Statistical analyses
Ⅲ. RESULTS AND DISCUSSION
1. Al stress-induced proteins in alfalfa roots tissues
2. Functional categorization of Al stress-induced rootprotein
Ⅳ. CONCLUSION
Ⅴ. ACKNOWLEDGEMENT
Ⅵ. REFERENCES

영어 초록

Aluminum (Al) is one of the major factors adversely affects crop growth and productivity in acidic soils. In this study, the effect of Al on plants in soil was investigated by comparing the protein expression profiles of alfalfa roots exposed to Al stress treatment. Two-week-old alfalfa seedlings were exposed to Al stress treatment at pH 4.0. Total protein was extracted from alfalfa root tissue and analyzed by two-dimensional gel electrophoresis combined with MALDI-TOF/TOF mass spectrometry. A total of 45 proteins differentially expressed in Al stress-treated alfalfa root tissues were identified, of which 28 were up-regulated and 17 were down-regulated. Of the differentially expressed proteins, 7 representative proteins were further confirmed for transcript accumulation by RT-PCR analysis. The identified proteins were involved in several functional categories including disease/defense (24%), energy (22%), protein destination (9%), metabolism (7%), transcription (5%), secondary metabolism (4%), and ambiguous classification (29%). The identification of key candidate genes induced by Al in alfalfa roots will be useful to elucidate the molecular mechanisms of Al stress tolerance in alfalfa plants.

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