Gel extraction & TA cloning A+레포트

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"Gel extraction & TA cloning A+레포트"에 대한 내용입니다.

목차

1. Title
2. Name
3. Purpose
4. Materials
5. Methods
6. Results
7. Discussion
8. References

본문내용

1. Gel extraction
- Important notes: Buffer provided in this kit contain irritants. Wear gloves and lab coat when handling these buffer.
1) Add 500㎕ of FADF buffer to the sample (300mg gel slice) and mix by vortexing.
- For >2% agarose gels, add 1000㎕ of FADF buffer. But we used 0.8% agarose gels, so we add 500㎕ of FADF buffer.
2) Incubate at 55℃ for 10 minutes and vortex the tube every 2 minutes until the gel slice dissolved completely.
- Make sure that the gel slice has been dissolved completely before proceed the next step and after gel dissolved, make sure that the color of sample mixture is yellow.
3) Cool down the sample mixture to room temperature. And place a FADF column into a collection tube.
4) Transfer 800㎕ of the sample mixture to the FADF column. Centrifuge at 13,000rpm for 30 seconds, then discard the flow-through.
- If the sample mixture is more than 800㎕, transfer all remaining samples too.

참고 자료

이병무, 『유전자 클로닝과 DNA 분석』, 월드사이언스(2017), 5-15, 50-58, 66-67, 94-99p.
이정주, 『유전학원론』, 월드사이언스(2013), 367-374p.
이종호, 『의생명과학 실험서 상권』, 라이프사이언스(2021), 154-161, 186-189p.
“다중클로닝 부위”, 분자·세포생물학백과, 한국분자·세포생물학회
“T4 DNA연결효소”, 생명과학대사전, 한국생물과학협회