HIV 클론항체의 생산

소개

"HIV 클론항체의 생산"에 대한 내용입니다.

목차

1. Introduction
2. Methods
3. Results
4. Discussion
5. Conclusion
6. References

본문내용

The antibodies have various functions such as opsonistion, complement activation, antibody dependent cell mediated cytotoxicity agglutination and degranulation and because of these, the antibodies have used for several purpose like vaccination and enzyme detection (Schroeder and Cavacini, 2010). The antibodies can have these functions because they have specific structure and these are classified into five groups, IgG, IgA, IgM, IgE and IgD (Schroeder and Cavacini, 2010). Each group has its specialized functions which are already mentioned. For commercial and medical purpose, the antibodies are artificially made and there are two outcomes which are known as monoclonal antibody and polyclonal antibody. The polyclonal antibody is a preparation containing many different antibodies even against the same antigen and typically produced by immunizing an animal and isolating the immunoglobulin fraction from the serum. However, the monoclonal antibody is a uniform clone of antibody molecules. All molecules in a monoclonal antibody population will have the same binding specificity, affinity and biological functions because monoclonal antibodies have the same amino acid sequences since they are produced by a population of identical cells (Anon, 2018).

참고자료

· Anon. (2017). Figure 4 HIV Western Blot. BS0505 Cellular and Immunological Methods Lecture Slides
· Lerner, E. (1981). How to make a hybridoma. Yale Journal of Biology Medicine, [online] 54(5), pp.387~402.
· Nyrhinen, T. (2000). Ethics in the laboratory examination of patients. Journal of Medical Ethics, 26(1), pp.54-60.
· Schroeder, H. and Cavacini, L. (2010). Structure and function of immunoglobulins. Journal of Allergy and Clinical Immunology, 125(2), pp.S41-S52.
· Tomita, M. and Tsumoto, K. (2011). Hybridoma technologies for antibody production. Immunotherapy, 3(3), pp.371-380.
· University of California. (2011). What kinds of HIV screening tests are available in the United States?.
· World Helth Organization (2010). WHO recommendations on the diagnosis of HIV infection in infants and children. 20, avenue Appia 1211 Geneva 27 Switzerland: World Health Organization Department of HIV/AIDS.