일반생물실험 랩 영어 레포트 7. 웨스턴 블롯 (Western Blotting)
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"일반생물실험 랩 영어 레포트 7. 웨스턴 블롯 (Western Blotting)"에 대한 내용입니다.
목차
1. Introduction
2. Materials
3. Methods
4. Results
5. Discussion
6. References
본문내용
Introduction
Blotting, in molecular biology and genetics, is a method of transferring proteins, DNA or RNA onto a carrier. In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly onto the membrane. After the blotting, the transferred proteins, DNA or RNA are then visualized by colorant staining, or specific labelling of some proteins. The latter is done with antibodies or hybridization probes that bind only to some molecules of the blot and have an enzyme bind to them. After proper washing, this enzymatic activity is visualized by incubation with proper reagent, rendering either a coloured deposit on the blot or a chemiluminescent reaction.
참고 자료
Hames, B.D. and Rickwood, D. Eds. (1990) Gel Electrophoresis of Proteins: a Practical
Approach, 2nd ed. Oxford University Press, New York.
Coligan, J.E., et al., Eds. (2002). Electrophoresis, In Current Protocols in Protein Science, pp. 10.0.1-10.4.36. John Wiley and Sons, Inc. New York.
