[A+ 연세대][2019 1학기 진행] 미생물및생물공학기초실험 Lab 8: Screening Natural Inhibitory Compounds on Melanin Biosynthesis 결과레포트

소개

2019년 1학기에 진행된 생명공학과 미생물및생물공학기초실험 과목에서 최종 성적 A+를 받은 결과레포트입니다. 결과레포트가 YSCEC을 이용하여 Turnitin 검수과정을 거치지 않고 메일과 하드카피 제출로 이루어지기 때문에 해당 레포트 내용을 조금씩만 변경하여 제출하시면 수월하게 A+를 받아가실 수 있을 것입니다. 글꼴은 맑은 고딕(본문) 혹은 a타이틀고딕(네이버에서 다운로드 가능)으로 작성하였습니다.

목차

1. Objective
2. Theory
3. Materials
4. Procedure
5. Result
6. Sample
7. Discussions
8. Reference

본문내용

[Objective]
Through the experiment, we are trying to understand the melanin biosynthesis pathway and also about screen inhibitors during the process of melanin biosynthesis.

[Theory]
For the theory of the experiment we studied Melanocytes and Tryrosinase Inhibition Assay.
First, melanin is produced through the process known as melanogenesis by Melanocytes. The process includes a cascade of enzymatic reactions like the following. First amino acid tyrosinase is oxidized and rearranged to DOPAchrome. Then DOPAchrome will be oxidized when there is oxygen resulting melanochrome, which eventually polymerizes and becomes melanin.
In the process of polymerization various enzymes are involved and tyrosinase is the enzyme that is rate-limiting. Functional tyrosinase requires the binding to L-DOPA, that is a cofactor and also a substrate. When L-DOPA binds its causes conformational

Next is Tyrosinase Inhibition Assay. Tyrosinase is the key enzyme while also being the rate limiting enzyme to catalyze the steps of melanogenesis.

참고자료

· “Experiment in Microbiology and Biochemical Engineering”, 이동우 외 7명 저, Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University
· Molecular Cell Biology, Lodish, et al, W.H. Freeman and Company, New York
· Microbiology with Diseases by Body System, 4th Ed. Robert Bauman, Pearson Higher Education
· Chemistry of Natural Antioxidants and Studies performed with different plants Collected in Mexico, Perez, Jorge Alberto Mendoza, and Tomas Alejandro FregosoAguilar. Intech.