고려대학교 미생물학 실험 8 Production of Indole & Reduction of Nitrate 결과

목차

I. Material
II. Experiment
III. Results
IV. Discussion
V. References

본문내용

I. Material
1) Production of indole
- 1% Tryptone broth
- Kovacs' reagent( or Ehrlich's reagent)
25mL의 concentrated HCl을 75ml의 Amyl alcohol에 넣어준다.
위의 용액에 5g의 p-dimethylamino-benzaldehyde를 녹인다.
한번 사용할 만큼만 분주하여 4℃에서 보관한다.
2) Reduction of nitrate
- Nitrate broth (/L)
Beef extract -------- 3g
Peptone ------------ 4g
Potassium nitrate ---- 1g
- Nitrite test reagents(solution A & B)
solution A : 0.8% sulfanilic acid in 5N acetic acid
solution B : 0.6% N,N-dimethyl-α-naphthylamine in 5N acetic acid
- Zinc dust

참고 자료

Wikipedia contributors. "Indole test." Wikipedia, The Free Encyclopedia. Wikipedia, The Free Encyclopedia, 14 Oct. 2010. Web. 18 Nov. 2010.
Wikipedia contributors. "Nitrate reductase test." Wikipedia, The Free Encyclopedia. Wikipedia, The Free Encyclopedia, 15 Apr. 2010. Web. 18 Nov. 2010.