The Phylogeny of Land Plants Inferred from 18s rDNA Sequences,Pushing the Limits of rDNA Signal

목차

1. Introduction
2. Materials & Methods
3. Results
4. Discussion
5. Conclusion

본문내용

Unclear relationship of many of the major lineages of land plants for understanding land plant phylogeny
 Ribosomal RNA or DNA sequences are used to reconstruct deep branches of evolutionary history

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Weighting schemes of land plants: reflect evolutionary pattern
Transition : transversion ratio is 1.9
Step matrix : to weight transitions half as much as transversions

Model of the secondary structure of rRNA for Glycine max : to estimate whether specific nucleotides should be considered components of stems or loops
All 1,869 nt mapped onto the secondary structure for G.max

Designation of bases as a stem base or a loop base : accurately classifies almost all nucleotides in plant 18S rDNA

Loops defined as being four bases or more in length : - loops of 4 nt , make up over 50% of the hairpin loops in 16S rRNA

The minimum size of 4 nt : correspond to patterns of base-paring in rRNA

Stem bases : a weight of 1,
Loop bases : a weight of 2

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