site directed mutagenesis

소개

1. site directed mutagenesis란?
(개념 및 구체적인 원리)
2. site directed mutagenesis protocols(구체적인 실험방법들)
3. site directed mutagenesis example.

목차

1. conception
2. principle
3. material and method
4. application of the method in study

본문내용

* conception
“ PROTEIN FUNCTION IS DETERMINED BY STRUCTURE”
1) Domain - structure and function in Protein
2) the major role part & motif
3) DNA sequence insertion, changes, deletion: activate the motif In vitro

2. Using E.coli DAM methylase – DNA methylation
1) Mutant strand synthesis
Perform thermal cycling to
- denathre DNA template
- anneal mutagenic primers containing desired mutation
- extend and incorporate primers with PfuUltra DNA polymerase

2) Dpnl digestion of temolate
Digest parental methylated and hemimethylated DNA wite Dpnl

참고자료

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