桂芍知母湯加味方이 골관절염에 미치는 영향 (Effect of Kyejakjimo-tangkami (Guìsháozhīmǔ-tāngjiāwèi) on Osteoarthritis)

서지정보

· 발행기관 : 한방재활의학과학회
· 수록지 정보 : 한방재활의학과학회지 / 23권 / 4호 / 39 ~ 57페이지
· 저자명 : 홍성민, 오민석

초록

Objectives The purpose of this study is to prove the effect of Kyejakjimotangkami(KMK)on osteoarthritis.
Methods We checked antioxidant activity and measured production of IL-1β, IL-6,TNF-α in RAW 264.7 cell after treat by KMK. Then we measured hind paw weight ofWister Rat with arthritis induced by MIA after KMK oral administration, checkedProstaglandin E2, IL-1β, IL-6, TNF-α, Osteocalcin, TIMP-1, MMP-9, LTB-4 in serum,ran histopathological test and μCT-arthrography.
Results 1. DPPH radical Scavenging was increased depend on concentration of KMKethanol extract in RAW 264.7 cell. 2. Production of NO was significantly decreased byKMK ethanol extract on concentration of 200μg/ml in RAW 264.7 cell. 3. Production ofIL-1β was significantly decreased by KMK ethanol extract on concentration of 200μg/ml. And Production of IL-6 ,TNF-α were significantly decreased KMK ethanol extract ofevery concentration in RAW 264.7 cell. 4. Result of checking hind paw weight when administeredKMK ethanol extract to Wister Rat with arthritis induced by MIA was significantlyhigher than control group and similar to normal group. 5. Production ofProstaglandin E2, IL-1β, Osteocalcin, TIMP-1, MMP-9 and LTB-4 in serum was significantlydecreased by KMK ethanol extract after administerd to Wister Rat with arthritisinduced by MIA. 6. In Hematoxylin & Eosin staining and Safranin-O staining, we could findinflammation of synovial cell, infiltration of macrophage and granulocyte and degenerationof cartilage and bone were decreased in comparison with control group. 7. When checkedcartilage volume to examine degree of cartilage degeneration using μCT-arthrography,volume of cartilage was increased in comparison with control group.
Conclusions Comparison of the results for this study showed that KMK ethanol extracthave anti-inflammatory effectiveness and can protect cartilage and bone. So we expectthat KMK can be used as a effective drugs for osteoarthritis.

영어초록

Objectives The purpose of this study is to prove the effect of Kyejakjimotangkami(KMK)on osteoarthritis.
Methods We checked antioxidant activity and measured production of IL-1β, IL-6,TNF-α in RAW 264.7 cell after treat by KMK. Then we measured hind paw weight ofWister Rat with arthritis induced by MIA after KMK oral administration, checkedProstaglandin E2, IL-1β, IL-6, TNF-α, Osteocalcin, TIMP-1, MMP-9, LTB-4 in serum,ran histopathological test and μCT-arthrography.
Results 1. DPPH radical Scavenging was increased depend on concentration of KMKethanol extract in RAW 264.7 cell. 2. Production of NO was significantly decreased byKMK ethanol extract on concentration of 200μg/ml in RAW 264.7 cell. 3. Production ofIL-1β was significantly decreased by KMK ethanol extract on concentration of 200μg/ml. And Production of IL-6 ,TNF-α were significantly decreased KMK ethanol extract ofevery concentration in RAW 264.7 cell. 4. Result of checking hind paw weight when administeredKMK ethanol extract to Wister Rat with arthritis induced by MIA was significantlyhigher than control group and similar to normal group. 5. Production ofProstaglandin E2, IL-1β, Osteocalcin, TIMP-1, MMP-9 and LTB-4 in serum was significantlydecreased by KMK ethanol extract after administerd to Wister Rat with arthritisinduced by MIA. 6. In Hematoxylin & Eosin staining and Safranin-O staining, we could findinflammation of synovial cell, infiltration of macrophage and granulocyte and degenerationof cartilage and bone were decreased in comparison with control group. 7. When checkedcartilage volume to examine degree of cartilage degeneration using μCT-arthrography,volume of cartilage was increased in comparison with control group.
Conclusions Comparison of the results for this study showed that KMK ethanol extracthave anti-inflammatory effectiveness and can protect cartilage and bone. So we expectthat KMK can be used as a effective drugs for osteoarthritis.

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