궤양성 대장염 동물모델에서 四味軟堅湯의 항염증 및 장내세균 효소활성 억제효과 (Effects of Samiunkyungtang on inflammation and fecal enzymes in ulcerative colitis animal model)

서지정보

· 발행기관 : 대한한의학회
· 수록지 정보 : 대한한의학회지 / 29권 / 3호 / 50 ~ 62페이지
· 저자명 : 류봉하, 김소연, 박재우

초록

Objectives:This study was aimed to find the effect of Samiunkyungtang on inflammation and microflora in an ulcerative colitis animal model.
Methods:We established four groups of normal, control, test 1, and test 2 and assigned 6 rats toeach group. The normal group was not treated by any process and fed by normal saline. The control & test groups were provided with 4% dextran sodium sulfate (DSS) treatment for 7 days. Samiunkyungtang extract was orally administered to test groups (test 1=25mg/kg, test 2 100mg/kg) 3 days after DSS treatment for 10 days. After DSS treatment finished, we sacrificed the mice and measured colon length and enzyme activities such as myeloperoxidase (MPO), alkine phosphatase (ALP), β-glucuronidase, β-glucosidase, chondroitinase, and tryptophanase.
Results:The colon lengths of test 1 and 2 groups were longer than the control group (p<0.05). Histologically, the crypts and superficial epitheliums of test 1 and 2 groups were regenerated. Goblet cells from all test groups were retrieved. The inflammatory biochemical marker, MPO and ALP activities in all test groups were highly reduced (p<0.01) compared to the control group. The activities of fecal bacterial enzymes in test groups such as β-glucuronidase, β-glucosidase, chondroitinase, and tryptophanase were reduced compared to the control group (p<0.01).
Conclusions:As a result of this experiment, Samiunkyungtang is considered to have an inhibitory effect on inflammation and fecal enzyme activity in DSS-induced colitis animal model. Our results indicate that Samiunkyungtang may possess therapeutic effect on the development of DSS-induced colitis.

영어초록

Objectives:This study was aimed to find the effect of Samiunkyungtang on inflammation and microflora in an ulcerative colitis animal model.
Methods:We established four groups of normal, control, test 1, and test 2 and assigned 6 rats toeach group. The normal group was not treated by any process and fed by normal saline. The control & test groups were provided with 4% dextran sodium sulfate (DSS) treatment for 7 days. Samiunkyungtang extract was orally administered to test groups (test 1=25mg/kg, test 2 100mg/kg) 3 days after DSS treatment for 10 days. After DSS treatment finished, we sacrificed the mice and measured colon length and enzyme activities such as myeloperoxidase (MPO), alkine phosphatase (ALP), β-glucuronidase, β-glucosidase, chondroitinase, and tryptophanase.
Results:The colon lengths of test 1 and 2 groups were longer than the control group (p<0.05). Histologically, the crypts and superficial epitheliums of test 1 and 2 groups were regenerated. Goblet cells from all test groups were retrieved. The inflammatory biochemical marker, MPO and ALP activities in all test groups were highly reduced (p<0.01) compared to the control group. The activities of fecal bacterial enzymes in test groups such as β-glucuronidase, β-glucosidase, chondroitinase, and tryptophanase were reduced compared to the control group (p<0.01).
Conclusions:As a result of this experiment, Samiunkyungtang is considered to have an inhibitory effect on inflammation and fecal enzyme activity in DSS-induced colitis animal model. Our results indicate that Samiunkyungtang may possess therapeutic effect on the development of DSS-induced colitis.

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