열처리한 겉보리 에탄올 추출물이 3T3-L1 지방세포와 HepG2 간세포의 지질 축적 억제에 미치는 영향 (Anti-adipogenic effect of roasted whole barley ethanol extract on 3T3-L1 adipocytes and HepG2 hepatocytes)

서지정보

· 발행기관 : 한국식품과학회
· 수록지 정보 : 한국식품과학회지 / 56권 / 5호 / 593 ~ 600페이지
· 저자명 : 배지윤, 이예진, 김미자

초록

The objective of this study was to investigate the anti-obesity activity of the ethanol extract of whole barley (WBE) roasted at 150, 190, and 230oC on 3T3-L1 adipocytes and oleic acid (OA)-induced HepG2 hepatocytes. The WBE inhibited fat accumulation in 3T3-L1 and OA-induced HepG2 as its roasting temperature increased. At 100 μg/mL of 190oC WBE, the expression of genes related to adipogenic differentiation in 3T3-L1, such as pparγ, ap2, scd1, and acc1, was reduced by 21, 68, 39, and 63%, respectively, compared to that in adipocytes. Likewise, 75 μg/mL of 190oC WBE significantly inhibited the expression of transcription factors including pparγ, c/ebp-α, and srebp-1c in OA-induced HepG2 (p<0.05). In 3T3-L1, at 75 μg/mL of 190oC WBE, the protein levels of C/EBP-α, SREBP-1C, AP2, and SCD1, decreased by 31, 24, 45, and 52%, respectively. Overall, 190oC WBE inhibited fat accumulation in 3T3-L1 and HepG2, showing its potential use as a natural anti-obesity functional material.

영어초록

The objective of this study was to investigate the anti-obesity activity of the ethanol extract of whole barley (WBE) roasted at 150, 190, and 230oC on 3T3-L1 adipocytes and oleic acid (OA)-induced HepG2 hepatocytes. The WBE inhibited fat accumulation in 3T3-L1 and OA-induced HepG2 as its roasting temperature increased. At 100 μg/mL of 190oC WBE, the expression of genes related to adipogenic differentiation in 3T3-L1, such as pparγ, ap2, scd1, and acc1, was reduced by 21, 68, 39, and 63%, respectively, compared to that in adipocytes. Likewise, 75 μg/mL of 190oC WBE significantly inhibited the expression of transcription factors including pparγ, c/ebp-α, and srebp-1c in OA-induced HepG2 (p<0.05). In 3T3-L1, at 75 μg/mL of 190oC WBE, the protein levels of C/EBP-α, SREBP-1C, AP2, and SCD1, decreased by 31, 24, 45, and 52%, respectively. Overall, 190oC WBE inhibited fat accumulation in 3T3-L1 and HepG2, showing its potential use as a natural anti-obesity functional material.

참고자료

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