오이 유묘와 과실을 활용한 검은별무늬병에 대한 저항성 품종 검정 방법 (Screening Methods for Resistant Cucumber Cultivars against Cucumber Scab Caused by Cladosporium cucumerinum Using Cucumber Fruits and Seedlings)

서지정보

· 발행기관 : 한국식물병리학회
· 수록지 정보 : 식물병 연구 / 22권 / 1호 / 18 ~ 24페이지
· 저자명 : 박소향, 홍성준, 심창기, 김민정, 박종호, 한은정, 박종원, 지형진, 김석철, 김용기

초록

This study was conducted to elucidate the cultural and pathogenic characteristics of Cladosporiumcucumerinum PT1 and resistance of 81 commercial cucumbers (Cucumis sativus). Cucumber leavesand fruits appeared as scab were collected from a plastic film house located in Pyeongtaek, Gyeonggiprovince,Korea in late March, 2015. A casual fungus was isolated from the diseased fruits on potatodextrose agar and it was identified as C. cucumerinum PT1 based on the morphological characteristics.
To find out the effect of wounding and fruit size on the development of cucumber scab, small(<10 cm long), medium (10 to 20 cm long), and large (>20 cm long, commercially mature fruit) sizecucumber fruits were harvested, C. cucumerinum PT1 pathogens were inoculated with a singledroplet of suspension (1×105 spores/ml) on wounded or unwounded cucumber fruits. Small fruitswere completely damaged with showing severe water-soaking symptoms and fast pathogen growthregardless of wounded or unwounded. Meanwhile slight water-soaking symptoms on medium andlarge size fruits occurred and disease development into plant tissues was observed only on woundedfruits. Disease resistance of 81 commercial cucumber cultivars was evaluated on third-stage seedlingsand small fruits by inoculating suspension (1×105 spores/ml) of C. cucumerinum PT1. As a result, miniand pickling cultivar groups were resistant, ‘Cheoeumcheoreom’ cultivar was symptomless and theother cultivars were resistant to medium resistant. On the other hand, most of cucumber cultivarsbelonging to the other groups were susceptible. Disease resistance of cucumber against cucumberscab was significantly different among cultivars and a few cucumber cultivars showed different diseaseresistant responses to two bioassay methods using seedlings and small fruits. Therefore, to screen scabresistance in cucumber, a test using both fruits and seedlings is advisable. We think that the selectedresistant cultivars can be used to control cucumber scab effectively under the farmhouse condition.

영어초록

This study was conducted to elucidate the cultural and pathogenic characteristics of Cladosporiumcucumerinum PT1 and resistance of 81 commercial cucumbers (Cucumis sativus). Cucumber leavesand fruits appeared as scab were collected from a plastic film house located in Pyeongtaek, Gyeonggiprovince,Korea in late March, 2015. A casual fungus was isolated from the diseased fruits on potatodextrose agar and it was identified as C. cucumerinum PT1 based on the morphological characteristics.
To find out the effect of wounding and fruit size on the development of cucumber scab, small(<10 cm long), medium (10 to 20 cm long), and large (>20 cm long, commercially mature fruit) sizecucumber fruits were harvested, C. cucumerinum PT1 pathogens were inoculated with a singledroplet of suspension (1×105 spores/ml) on wounded or unwounded cucumber fruits. Small fruitswere completely damaged with showing severe water-soaking symptoms and fast pathogen growthregardless of wounded or unwounded. Meanwhile slight water-soaking symptoms on medium andlarge size fruits occurred and disease development into plant tissues was observed only on woundedfruits. Disease resistance of 81 commercial cucumber cultivars was evaluated on third-stage seedlingsand small fruits by inoculating suspension (1×105 spores/ml) of C. cucumerinum PT1. As a result, miniand pickling cultivar groups were resistant, ‘Cheoeumcheoreom’ cultivar was symptomless and theother cultivars were resistant to medium resistant. On the other hand, most of cucumber cultivarsbelonging to the other groups were susceptible. Disease resistance of cucumber against cucumberscab was significantly different among cultivars and a few cucumber cultivars showed different diseaseresistant responses to two bioassay methods using seedlings and small fruits. Therefore, to screen scabresistance in cucumber, a test using both fruits and seedlings is advisable. We think that the selectedresistant cultivars can be used to control cucumber scab effectively under the farmhouse condition.

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