Cytokine Production in Canine PBMC after Bartonella Henselae Infection

서지정보

· 발행기관 : 한국임상수의학회
· 수록지 정보 : 한국임상수의학회지 / 27권 / 4호 / 311 ~ 314페이지
· 저자명 : 최은화, 구혜정, 박용호, 윤화영, 이종화

초록

Bartonella henselae is the causative agent of cat scratch disease. Although cats are the main zoonotic reservoirs of Bartonella spp., unusual cases of cat scratch disease caused by a domestic dog scratch have been recently reported. For the in vivo B. henselae infection, eight dogs were inoculated intradermally with 2 × 108CFU of B. henselae Houston-1 suspended in 1 ml of phosphate buffered saline on day 0 and subsequent injections of the same amount given intradermally on days 21, 28, 36, 58 and 64. After in vivo canine B. henselae infection was confirmed by nested PCR, the IFN-γ levels of the culture supernatant of PBMC stimulated with B. henselae was significantly higher in the B. henselae-PCR positive group than the B. henselae-PCR negative group. Our results showed that the canine immune responses against B. henselae were different from those of cats. Th1 activation by B. henselae stimulation was characterized in dog peripheral blood mononuclear cells, whereas Th2 activation was reported in B. henselaeinfected cats.

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