Tyrosinase Inhibitory Activity of Rosmarinic Acid on Human Skin Melanoma Cells Treated with Hydrogen Peroxide (Tyrosinase Inhibitory Activity of Rosmarinic Acid on Human Skin Melanoma Cells Treated with Hydrogen Peroxide)

서지정보

· 발행기관 : 한국실험동물학회
· 수록지 정보 : Laboratory Animal Research / 25권 / 2호 / 95 ~ 100페이지
· 저자명 : Jeong-Ran Kang, Sun-Mi Yoo

초록

The purpose of this study was to evaluate the tyrosinase inhibitory activity of rosmarinic acid on the
cultured human skin melanoma cells damaged by reactive oxygen species (ROS). The cytotoxicity effect
was analyzed by MTT assay for cell viability, tyrosinase inhibitory activity after treating the cells with or
without hydrogen peroxide (H2O2) for 1-10 hours. Light microscopic study was also carried out for
morphological changes of the cells and tyrosinase activities. H2O2 decreased the cell viability in the
cultured human melanoma cells in a dose- and time-dependent manner after cells had been treated with
20-80 μM of H2O2 for 7 hours. IC50 value of H2O2 by the MTT assay was 80 μM. Rosmarinic acid
increases cell viability, inhibitory activity of tyrosinase. In light microscopic study, the number of human
skin melanoma cells treated with H2O2 was decreased compared with control, and H2O2 activated
tyrosinase activity, which was observed through darkened cell color (mid-activity, +++). However, colors
of cells treated with Rosmarinic acid changed from white to brown in cell staining due to tyrosinase
activity (moderate-activity, ++). The treatment of the cells with H2O2 shows highly toxic effects on
cultured human skin melanoma cells. Rosmarinic acid increases cell viability and tyrosinase inhibitory
activity in the cells treated with H2O2. It is considered that Rosmarinic acid shows tyrosinase inhibitory
effect on ROS such as H2O2.

영어초록

The purpose of this study was to evaluate the tyrosinase inhibitory activity of rosmarinic acid on the
cultured human skin melanoma cells damaged by reactive oxygen species (ROS). The cytotoxicity effect
was analyzed by MTT assay for cell viability, tyrosinase inhibitory activity after treating the cells with or
without hydrogen peroxide (H2O2) for 1-10 hours. Light microscopic study was also carried out for
morphological changes of the cells and tyrosinase activities. H2O2 decreased the cell viability in the
cultured human melanoma cells in a dose- and time-dependent manner after cells had been treated with
20-80 μM of H2O2 for 7 hours. IC50 value of H2O2 by the MTT assay was 80 μM. Rosmarinic acid
increases cell viability, inhibitory activity of tyrosinase. In light microscopic study, the number of human
skin melanoma cells treated with H2O2 was decreased compared with control, and H2O2 activated
tyrosinase activity, which was observed through darkened cell color (mid-activity, +++). However, colors
of cells treated with Rosmarinic acid changed from white to brown in cell staining due to tyrosinase
activity (moderate-activity, ++). The treatment of the cells with H2O2 shows highly toxic effects on
cultured human skin melanoma cells. Rosmarinic acid increases cell viability and tyrosinase inhibitory
activity in the cells treated with H2O2. It is considered that Rosmarinic acid shows tyrosinase inhibitory
effect on ROS such as H2O2.

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