Morphological Changes of the Sensory Neurons in the Peripheral Neuropathy of Rat Tibial Nerve Using WGA-HRP Tracing Method

서지정보

· 발행기관 : 대한체질인류학회
· 수록지 정보 : 해부·생물인류학 / 22권 / 1호 / 85 ~ 94페이지
· 저자명 : 김지현, 채옥희, 한의혁

초록

Neuropathy is a general term referring to disorders of nerves, and produces when the nerves are damaged. It
is characterized by spontaneous pain, allodynia and hyperalgesia. The purpose of present study is to observe the number
of WGA-HRP (wheat germ agglutinin-horseradish peroxidase) labelded sensory neurons of DRG (dorsal root ganglia),
and distributions according to cell size of sensory neuron in tibial nerve ligation model (NLM).
The tibial nerve ligation was performed with 3-0 silk by the application of three tight ligatures at the mid-thigh level.
In the neuropathy model of rat tibial nerve ligation, morphological changes of sensory neurons in DRG were observed using WGA-HRP.
Rats of NLM showed the neuropathic behaviors. Rats were shown guarding affected limb and limping. Their toes and
ankle joint of operated limb were hyperflexed. Under light microscopy, tibial nerve showed degeneration of axons in
NLM. In control and NLM, labeled sensory neurons of tibial nerve distributed L4 and L5 DRG. In control group, the
labeled sensory neurons were round or oval in shape. They were large and small cells, and mixed pattern. Total number
of labeled sensory neurons in NLM decreased significantly from control group. The number of labeled sensory neurons
in L4 and L5 DRG decreased significantly from control group. Labeled large and small cells decreased significantly from control group. Present study may serve as the basic information about the changes of DRG sensory neurons in NLM.

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