ICR 마우스 모델을 이용한 녹용 추출물의 생화학적 평가 및 급성 경구 독성을 포함한 세포 독성 효과

서지정보

· 발행기관 : 한국식품위생안전성학회
· 수록지 정보 : 한국식품위생안전성학회지 / 38권 / 6호
· 저자명 : 칠라칼라 라마크리시나, 문현정, 이환, 이동성, 정선희

목차

Materials and Methods
Deer velvet powder
General ingredients
Heavy metals and residual pesticides analysis invelvet-extracted samples
Bioactive compounds analysis using HPLC andUPLC/QTOF-HRMS method
Evaluation of cytotoxicity
Deer velvet antler samples in vivo treatment foranalyzing acute toxicity
Toxicity studies
Microbiological analysis of velvet extract samples
Statistical analysis
Results and discussion
Chemical composition and mineral contents of raw,dried, and freeze-dried antler extracts
Uronic acid, sulfated-GAGs, sialic acid, and collagencontents
Bioactive compounds analysis
Analysis of residual pesticides and heavy metalconcentrations in velvet antler extracts
Cytoprotective effect of raw, dried, and freeze-driedantler extract on HT22, BV2, RAW264.7, and HaCaTcells
Acute toxicity in ICR mice model
Microbiological analysis of deer velvet extracts
Acknowledgments
국문요약
Conflict of interests
ORCID

영어초록

Velvet antler is widely used as a traditional medicine, and numerous studies have demonstrated its tremendous nutritional and medicinal values including immunity-enhancing effects. This study aimed to investigate different deer velvet extracts (Sample 1: raw extract, Sample 2: dried extract, and Sample 3: freeze-dried extract) for proximate composition, uronic acid, sulfated glycosaminoglycan, sialic acid, collagen levels, and chemical components using ultra-performance liquid chromatography-quadrupole-time-of-light mass spectrometry. In addition, we evaluated the cytotoxic effect of the deer velvet extracts on BV2 microglia, HT22 hippocampal cells, HaCaT keratinocytes, and RAW264.7 macrophages using the cell viability MTT assay. Furthermore, we evaluated acute toxicity of the deer velvet extracts at different doses (0, 500, 1000, and 2000 mg/kg) administered orally to both male and female ICR mice for 14 d (five mice per group). After treatment, we evaluated general toxicity, survival rate, body weight changes, mortality, clinical signs, and necropsy findings in the experimental mice based on OECD guidelines. The results suggested that in vitro treatment with the evaluated extracts had no cytotoxic effect in HaCaT keratinocytes cells, whereas Sample-2 had a cytotoxic effect at 500 and 1000 μg/mL on HT22 hippocampal cells and RAW264.7 macrophages. Sample 3 was also cytotoxic at concentrations of 500 and 1000 μg/mL to RAW264.7 and BV2 microglial cells. However, the mice treated in vivo with the velvet extracts at doses of 500–2000 mg/kg BW showed no clinical signs, mortality, or necropsy findings, indicating that the LD50 is higher than this dosage. These findings indicate that there were no toxicological abnormalities connected with the deer velvet extract treatment in mice. However, further human and animal studies are needed before sufficient safety information is available to justify its use in humans.

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