병풀 추출물이 첨가된 음료 시제품의 장내 면역조절 효과와 지표물질

서지정보

ㆍ발행기관 : 한국식품영양학회 ㆍ수록지정보 : 한국식품영양학회지 / 36권 / 6호
ㆍ저자명 : 김연숙, 신현영, 구자평, 하은지, 정원비, 정미연, 유광원

목차

서 론
재료 및 방법
1. 병풀 추출물 함유 음료 시제품의 제조
2. 세포주 및 배양조건
3. 장 상피세포(Caco-2)를 이용한 항염증 활성
4. 대식세포주(RAW 264.7)를 이용한 항염증 활성
5. HPLC-DAD를 이용한 주요 triterpenoid의 정량 분석
6. 통계처리
결과 및 고찰
1. 병풀 추출물 함유 음료 시제품 3종의 세포주 독성 평가
2. 장 상피세포주(Caco-2)를 이용한 장 염증 억제 활성
3. 대식세포주(RAW 264.7)에서의 염증 억제 활성
4. 병풀 추출물이 함유된 음료의 주요 triterpenoid 분석
요약 및 결론
감사의 글
References

영어 초록

To produce an intestinal immunomodulatory beverage containing Centella asiatica extract (CAE), three types of CAE-added beverage prototypes were prepared, and their immunomodulatory activities and marker compounds were analyzed. As a result of the cytotoxicity assessment, all the beverages did not show significant toxicity compared to the control group. Next, the immunomodulatory activities of the beverage prototype were evaluated using the inflammatory model of IL-1β-induced intestinal epithelial cell line. All the samples significantly reduced the production of IL-6, IL-8, and MCP-1 in a CAE concentration-dependent manner. In addition, CAE-added beverages inhibited NO, IL-6, and IL-12 production in LPS-induced RAW 264.7 cells. When the major triterpenoids, as marker compounds for the production of CAE-added beverages, were analyzed by HPLC-DAD, only asiaticoside was detected beyond the limit of quantification, while madecassoside, madecassic acid, and asiatic acid were not detected. The amounts of asiaticoside in CAE-added beverage prototypes were confirmed in No. 1 (19.39 μg/mL), 2 (19.25 μg/mL), and 3 (19.98 μg/mL). In conclusion, the results of this study suggested that CAE-added beverage prototypes induced immunomodulatory effects in the intestinal inflammatory cell line models and asiaticoside could be used as a marker compound for CAE-added beverage production.

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