바이오마커로의 PTTG1의 발현과 구강 편평세포 암종의 침윤 및 성장에 미치는 영향

서지정보

· 발행기관 : 대한구강악안면병리학회
· 수록지 정보 : 대한구강악안면병리학회지 / 47권 / 5호
· 저자명 : 이연준, 박경원, 김시현, 박수연, 최종호

목차

Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
1. Data sources
2. Detection of differentially expressed genes(DEG) analysis
3. Validation of risk score model
4. Cell culture
5. Quantitative real-time polymerase chainreaction (qRT-PCR) analysis
6. 5-Ethynyl-2′-deoxyuridine (EdU) assay
7. Migration and invasion assay usingTranswell assays
8. Statistical analysis
Ⅲ. RESULTS
1. PTTG1 expression is elevated in tumor patientsand is associated with cell cycle mechanisms.
2. PTTG1 overexpression affects overallsurvival (OS) in OSCC, but not in HNSC.
3. The knockdown of PTTG1 reduces theproliferation metastasis ability of OSCC cell lines.
Ⅳ. DISCUSSION

영어초록

Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer, and it has been steadily increasing in worldwide. Pituitary tumor transforming gene 1 (PTTG1) has been known as oncogene in a verity of cancers. Nevertheless, the expression and role of PTTG1 in OSCC progression remains largely unexplored. In this study, clinical datasets were analyzed to assess the genetic impact of PTTG1 on OSCC progression and to identify its functional roles in OSCC cell lines. We analyzed the expression of PTTG1 in head and neck squamous cell carcinoma (HNSC) and OSCC using databases form the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO). To investigate the effect of PTTG1 on proliferation and migration abilities in OSCC cell lines, following the knockdown of PTTG1 in HSC-2 and SCC-9 cell lines, we analyzed the proliferation and metastatic abilities of OSCC cells using EdU and Boyden chamber assays. Our database analysis revealed that PTTG1 was significantly overexpressed in tumor tissues compared to normal tissues. Moreover, its expression correlated with clinical parameters of OSCC. In vitro experiments demonstrated that depletion of PTTG1 suppressed the ability of cell proliferation and migration in both HSC-2 and SCC-9 cell lines. In conclusion, our study suggests that PTTG1 may act as an oncogene in OSCC. These findings provide new insights into the mechanisms and clinical implications of PTTG1 expression in OSCC patients.

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