Effect of dyslipidemia on vascular smooth muscle cell proliferation in a porcine coronary restenosis model

서지정보

· 발행기관 : 충북대학교 동물의학연구소
· 수록지 정보 : Journal of Biomedical and Translational Research / 20권 / 4호
· 저자명 : Kang Jin Jeong, Philyong Kang, Hwal-Yong Lee, Hee Chang Son, Sung Hyun Park, Won Seok Choi, Bon Sang Koo, Chang-Yeop Jeon, Seug Ho Baek, You Jung An, Hyun Kuk Kim, Sung Soo Kim, Jun Kyu Park, In Ho Bae, Dae Sung Park, Jae Won Shim, Jae Un Kim, Nan Yeol Kim, Sun-Uk Kim, Kyung Seob Lim, Myung Ho Jeong

목차

Introduction
Materials and Methods
Ethics statement for animal experiments
Diet
Serum cholesterol analysis
Animal preparation and stent implantation
Follow-up, sacrifice, and sampling
Histopathological analysis
Evaluation of injury score
Evaluation of inflammation scores and fibrin score
Statistical analysis
Results
Procedure outcomes after balloon angioplasty and stent implantation
Alteration in serum lipid profile
Difference in vessels after balloon angioplasty
Histopathologic analysis of stented arteries
Alteration in body weight
Discussion
Conclusion
References

영어초록

This study aimed to examine the effect of a mild elevation in serum cholesterol level in a porcine coronary overstretch restenosis model using a balloon angioplasty catheter or drug-eluting coronary stent. Pigs were divided into two groups and were fed a commercial normal diet (CND, n = 4) or a high-fat diet (HFD, n = 4) for 5 weeks. Coronary overstretch injury by balloon angioplasty or stent implantation was induced in the left anterior descending and left circumflex artery after 1 week of feeding. Histopathological analysis was performed at 4 weeks after coronary injury. During the experiment, the total cholesterol level in the HFD group increased by approximately 44.9% (from 65.9 ± 3.21 mg/dL at baseline to 95.5 ± 9.94 mg/dL at 5 weeks). The lumen area in the CND group was reduced in comparison with that in the HFD group after balloon angioplasty. After stent implantation, the injury score showed no significant difference. There were significant differences in the neointimal area (2.7 ± 0.33 mm2 in the CND group vs. 3.3 ± 0.34 mm2 in the HFD group, p<0.05), lumen area (2.6 ± 0.54 mm2 in the CND group vs. 2.0 ± 0.33 mm2 in the HFD group, p<0.05), and percent area stenosis (52.0 ± 7.96% in the CND group vs. 62.4 ± 5.15% in the HFD group, p<0.05). Body weight change was not different between the two groups. Increased serum cholesterol level activated vascular smooth muscle cell proliferation in the porcine coronary overstretch model.

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