Diethylnitrosamine 에 의한 계배 간 조직 손상 및 지질 성분의 변화

서지정보

· 발행기관 : 한국식품위생안전성학회
· 수록지 정보 : 한국식품위생안전성학회지 / 14권 / 1호
· 저자명 : 박정현, 정덕화, 강진순, 강성조

영어초록

Diethylnitrosamine (DEN) is known as a potential hepatic carcinogen by single administration. This study was designed to measure the effects of DEN-induced cell damage on the triglyceride and cholesterol concentration in the liver, excluding dietary effects. Fertilized chicken eggs, 10 days before hatching, were randomly divided into three groups (n=20) and each egg was injected 10 ul of corn oil (vehicle control), 5 ug of DEN/10 ul and 10 ug of DEN/10 ul into yolk via air sac. After 48 hr and 96 hr incubation, the damage of the chick-embryo liver cell was investigated by electron microscopy and by measuring the concentration of lipid components (total cholesterol, free cholesterol, phospholipid and triglyceride). For eggs administered 10 ug of DEN and incuvated 96 hr, in hepatocyte, the nucleus membrane was roughed, the size of nucleolus was apparently increased and euchromatin was accumulated. Mitochondria were condensed and cristae, located mitochondrial inner membrane, were obscured. Additionally, the levels of triglyceride and cholesterol classes were significantly increased depend on the amount of DEN and incubation time. Especially, triglycerides were notably increased in the group treated with 10 ug DEN at 96 hr, but phospholipids, component of cell membrane, were decreased with significance. As a conclusion, carcinogen induced hepatic lesion was correlated with the changes in lipid component of liver.

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