Glucosinolate Content Varies and Transcriptome Analysis in Different Kale Cultivars (Brassica oleracea var. acephala) Grown in a Vertical Farm
(주)코리아스칼라
- 최초 등록일
- 2023.04.03
- 최종 저작일
- 2022.10
- 11페이지/ 어도비 PDF
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서지정보
ㆍ발행기관 : 한국생물환경조절학회
ㆍ수록지정보 : 생물환경조절학회지(구 시설원예ㆍ식물공장) / 31권 / 4호
ㆍ저자명 : Thi Kim Loan Nguyen, Ga Oun Lee, Jung Su Jo, Jun Gu Lee, Shin-Woo Lee, Ki-Ho Son
목차
Abstract
Introduction
Materials and Methods
1. Plant materials
2. Growth conditions
3. Glucosinolate analysis
4. RNA Preparation
5. RNA-Seq analysis
6. Statistical analysis
Results
1. Growth characteristics
2. Quantification of individual glucosinolatecontent in the three kale cultivars
3. Illumina sequencing and de novo transcriptomeassembly of the three kale cultivars
4. Identification of glucosinolate andphenylpropanoid synthesis genes from thetranscriptomes of the three kale cultivars
Discussion
Acknowledgments
Literature Cited
영어 초록
Kale (Brassica oleracea var. acephala) is one of the most frequently consumed leafy vegetables globally, as it contains numerous nutrients; essential amino acids, phenolics, vitamins, and minerals, and is particularly rich in glucosinolates. However, the differences in the biosynthesis of glucosinolates and related gene expression among kale cultivars has been poorly reported. In this study, we investigated glucosinolates profile and content in three different kale cultivars, including green (‘Man-Choo’ and ‘Mat-Jjang’) and red kale (‘Red-Curled’) cultivars grown in a vertical farm, using transcriptomic and metabolomic analyses. The growth and development of the green kale cultivars were higher than those of the red kale cultivar at 6 weeks after cultivation. High-performance liquid chromatography (HPLC) analysis revealed five glucosinolates in the ‘Man-Choo’ cultivar, and four glucosinolates in the ‘Mat-Jjang’ and ‘Red-Curled’ cultivars. Glucobrassicin was the most predominant glucosinolate followed by gluconastrutiin in all the cultivars. In contrast, other glucosinolates were highly dependent to the genotypes. The highest total glucosinolates was found in the ‘Red-Curled’ cultivar, which followed by ‘Man-Choo’ and ‘Mat-Jjang’. Based on transcriptome analysis, eight genes were involved in glucosinolate biosynthesis. The overall results suggest that the glucosinolate content and accumulation patterns differ according to the kale cultivar and differential expression of glucosinolate biosynthetic genes.
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