가축사료 및 그 원료에 대한 DNA 추출방법의 효율 비교

서지정보

ㆍ발행기관 : 한국국제농업개발학회 ㆍ수록지정보 : 한국국제농업개발학회지 / 28권 / 2호
ㆍ저자명 : 신공식, 우희종, 임명호, 이진형, 친 양, 박순기, 권택윤, 조현석

목차

재료 및 방법
시험재료
DNA의 추출
DNA의 농도 및 순도 확인
Primer 제작
PCR 분석
결과 및 고찰
시료특성 및 primer 선발
Genomic DNA 추출방법 비교
선발 DNA 추출방법에 대한 PCR 분석
적 요
REFERENCES

영어 초록

Recently, in Korea various kinds of genetically modified (GM) crops have been imported and used as a raw material to manufacture foods and livestock feeds, but the different social concerns about the benefits and the potential risks of GM crops are being shown with a different reaction from the public. Thus a persistent management is required for the safe utilization of genetically modified organism (GMO). PCR analysis of transgene into crop is generally performed for the efficient post management of GMOs. The most important prerequisite for the application of nucleic acid detections is to decide the effective DNA-extraction methods. Particularly, in the case of processed feeds, the nucleic acids of which may be damaged by heating, high pressure, pH treatments, fermentation, etc. in processing, DNA must be extracted with high sensitivity from the samples to perform the PCR successfully. In this study, seven of DNA-extraction methods used commercially and non-commercially were compared with respect to the yields and quality of DNA extracted from livestock feeds and those crop materials. Amounts of genomic DNA obtained from the extraction methods varies according to feed configurations and crop materials. The DNA yield and uniformity of samples extracted with PG, CTAB, and QF method is greater than that obtained from other extraction methods. In the DNA integrity of the selected extraction methods, PCR analysis showed distinct amplifications and similar patterns in detecting crop endogenous genes and GMO genes. These results would be applicable for the selection of an adequate DNA-extraction method in extracting processed feeds and/or crop materials.

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