유전자 변형 비타민E 강화콩의 환경 영향 평가 : 비타민E 강화콩의 급이가 물벼룩(Daphnia magna)에 미치는 영향

서지정보

ㆍ발행기관 : 한국국제농업개발학회 ㆍ수록지정보 : 한국국제농업개발학회지 / 28권 / 2호
ㆍ저자명 : 오성덕, 윤도원, 손수인, 이범규, 이기종, 장안철

목차

재료 및 방법
시료 제조
Genomic DNA 추출 및 도입유전자 Polymerase chainreaction (PCR) 분석.
환경 방출 조건에 따른 비타민 E 강화콩의 목적 단백질함량 분석
비타민 E 강화콩의 PAT 단백질 분석
급성독성 평가를 위한 물벼룩 배양 조건
물벼룩 급성독성 시험의 비타민 E 강화콩과 Willams 82처리 조건
물벼룩 급성독성 평가를 위한 조사항목
결과 및 고찰
비타민 E 강화콩의 분자생물학적 분석
비타민 E 강화콩의 노출 조건과 시기별 목적 단백질 함량분석
물벼룩 급성독성 시험 용수의 수질 분석
비타민 E 강화콩과 Willams 82의 처리농도에 따른 물벼룩의 유영 저해 분석
비타민 E 강화콩과 Willams 82의 급이에 의한 물벼룩의급성 독성
적 요
REFERENCES

영어 초록

The vitamin E enhanced transgenic soybean was developed by introducing a perilla γ- tocopherol methyltransferase gene (γ-TMT) under the control of pea vicilin promoter and a selection marker, phosphinothricin acetyltransferase (PAT) gene. With regard to the potential problems of safety, the non-target organism evaluation is required as an essential element for the environmental risk assessment of genetically modified (GM) crops. We studied the effects of the vitamin E enhanced transgenic soybean feeding on survival of Daphnia magna which is commonly used as a model organism in ecotoxicological studies. The Daphnia magna was fed on vitamin E enhanced transgenic soybean and non-genetically modified (non-GM) soybean (Willams 82) at 0, 1,000, 1,800, 3,240, 5,830, 10,500 and 20,000 mg/L concentrations, respectively. The GM soybean used for the test was confirmed to have the γ-TMT/PAT gene expression by the enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) analysis. The results showed that there was no significant differences between cumulative immobilities of Daphnia magna fed on GM soybean and non-GM soybean. The 48hr-EC50 values showed no significant differences between GM soybean (2,416 mg/L) and non-GM soybean (2,408 mg/L). The results of this study suggested that there was no significant differences in toxicity for Daphnia magna between GM soybean and non-GM counterpart.

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