Tetracycline 유도적인 RNAi System을 이용한 생쥐 성장 관련 유전자의 발현 억제
(주)코리아스칼라
- 최초 등록일
- 2016.04.02
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- 2014.09
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 38권 / 3호
ㆍ저자명 : 손혜진, 구본철, 권모선, 이영만, 김태완
목차
ABSTRACT
서 론
실험 방법
shRNA 합성
생쥐 shRNA를 도입한 Tet Vector System의 유전자 발현의 Knock-Down 효과 검정
결 과
Tetracycline 유도적인 Retrovirus Vector System을 이용한 mIGF-1i의 Knock-Down 효과 검정
Tetracycline 유도적인 Retrovirus Vector System을 이용한 mGHRi의 Knock-Down 효과 검정
단백질 수준에서의 mIGF-1i와 mGHRi의 Knock-Down 효율 양상 확인
고 찰
인용문헌
영어 초록
In this study, to further understand the mechanism of animal growth and to develop a miniature transgenic animal model, we constructed and tested tetracycline-inducible RNAi system using shRNA targeting the mRNA of mouse insulin-like growth factor (mIGF-1) or mouse growth hormone receptor (mGHR) gene. Quantitative real-time PCR analysis of mouse liver cell (Hepa1c1c7) cells transfected with these vectors showed 85% or 90% of expression inhi-bition effect of IGF-1 or GHR, respectively. In ELISA analysis, the protein level of IGF-1 in the cells expressing the shRNA targeting IGF-1 mRNA was reduced to 26% of non-transformed control cells. Unexpectedly, in case of using shRNA targeting GHR, the IGF-1 protein level was decreased to 75% of control cells. Further experiments are needed to explain the lower interference effect of GHR shRNA in IGF-1 protein. Accumulated knowledge of this approach could be applicable to a variety of related biological area including gene function study, gene therapy, development of miniature animals, etc.
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