번식주기의 단계별로 회수한 고양이 난자의 체외발생에 미치는 요인에 관한 연구

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 28권 / 1호
ㆍ저자명 : 전연화, 이명헌, 김상근

목차

I. INTRODUCTION II. MATERIALS AND METHODS 1. Oocyte recovery and culture 2. Pretreatment of sperm 3. In vitro gertilization 4. Assessment of fertilization and developmental rate 5. Statistical analysis III. RESULTS AND DISCUSSION 1. Developmental rates of ovum collected at different reproductive stages 2. Developmental rates at different culture conditions 3. IVF time and fertilization rate 4. The relationship between morphology and developmental rate 5. The relationship between fertilization rate and reproductive cycles IV. REFERENCES

영어 초록

The study was carried out to investigate the effects of morphology, reproductive cycle, incubation time and activation of oocytes on in vitro maturation of cat oocytes and development of IVM/IVF embryos. The results were summarized as follows: 1. When recovered from ovaries collected at different stages of the reproductive cycle (inactive, follicular and luteal stage), the developmental rates of oocytes to GV and MI stage were 72.5% and 27.5%, 57.5% and 7.5%, 62.5% and 17.5%, respectively. 2. The developmental rates of oocytes with cumulus cells to GV and MI stage in different conditions of incubation (5% CO₂, 95% O₂ and 10% CO₂, 90% O₂) were 70.0% and 27.5%, 52.5% and 20.0%, 55.0% and 12.5%, respectively. 3. The developmental rates to GV and MI oocytes when cultured at different time of incubation (17∼20, 21∼24, 25∼28 and 29∼32 h) were 67.5% and 20.0%, 67.5% and 30.0%, 62.5% and 22.5%, 65.0% and 15.0%, respectively. 4. The fertilization and cleavage rates of freshly collected oocytes with and without cumulus cells were 72.5% and 25.0%, 37.5% and 7.5%, respectively. The rates were greater in oocytes with cumulus cells than those without cumulus cells. 5. The fertilization and cleavage rates of oocytes recovered from ovaries collected at different stages of the reproductive cycle (inactive, follicular and luteal stage) were 75.0% and 25.0%, 40.0% and 7.5%, 50.0% and 15.0%, respectively.

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