Liposome을 매개로 한 태아 및 웅성 생식선으로의 전기적 유전자 도입

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 28권 / 1호
ㆍ저자명 : 최승철, 최승규, 최성식, 김선욱, 조나나, 정주영, 박창식, 이성호, 이상호

목차

I. INTRODUCTION II. MATERIALS AND METHODS 1. Preparation of plasmid DNA and DNA/ liposome complexes 2. Animals and in vivo gene transfer 3. Culture and gene transfection 4. X-gal staining of transfected cells or tissues III. RESULTS 1. lsolation and Characterization of Gonadal Sex from Porcine Fetuses 2. Expression of Transgene Introduced into the Gonads 3. In vitro Gene Expression in Porcine Primary Gonad Cells IV. DISCUSSION V. REFERENCES

영어 초록

Gene delivery is one of the keen interests in animal industry as well as research on gene functions. Some of the in vivo gene delivery techniques have been successively used in various tissues for the gene therapy and transgenesis. Despite intensive efforts, it still remains to overcome problems of limited local and regional administration and low transgene expression. To improve the efficiency of gene delivery, a new procedure was tested. We injected exogenous DNA containing LacZ into the female or male gonads and then pulsed electric field. Electroporated gonads showed positive X-gal staining in many seminiferous tubules of the porcine fetal gonads. Exogenously introduced LacZ genes were also expressed in female porcine gonad. In addition, we demonstrated efficient gene delivery in gonad of adult mouse. Furthermore, we succeed to generate genetically modified germline cells showing GFP and positive X-gal signals. The results suggest that the newly developed gene delivery is an effective way of in vivo transfection in mammals. The developed gene delivery procedure should be useful in producing transgenic animals when combined with primary cell culture and nuclear transplantation.

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